CA2 Protein (AA 1-260)

Catalog No. ABIN667740

This Recombinant CA2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CA2 Protein (AA 1-260) (ABIN667740)

Target: CA2 (Carbonic Anhydrase II (CA2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-260

Sequence: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

Characteristics: Carbonic anhydrase 2 , 1-260aa, Human, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity is > 7,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase 2/CA2

Background: Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. Recombinant human CA2 was expressed in E. coli and purified by using conventional chromatography.

Molecular Weight: 29.2 kDa (260aa) confirmed by MALDI-TOF

NCBI Accession: NP_000058